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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
14/02/2011 |
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Data da última atualização: |
19/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. |
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Afiliação: |
MARCOS ROBERTO CHIARATTI, USP/PIRASSUNGA; CHRISTINA RAMIRES FERREIRA, USP/PIRASSUNGA; FELIPE PERECIN, USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; JULIANO RODRIGUES SANGALLI, USP/PIRASSUNUGA; LÍGIA GARCIA MESQUITA, USP/PIRASSUNGA; JÚLIO CÉSAR DE CARVALHO BALIEIRO, USP/PIRASSUNUNGA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL/SAINT-HYACINTHE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL; FLÁVIO VIEIRA MEIRELLES, USP/PIRASSUNUNGA. |
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Título: |
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011. |
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DOI: |
https://doi.org/10.1016/j.rbmo.2010.10.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. MenosOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to ... Mostrar Tudo |
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Palavras-Chave: |
Embryo; Ethidium bromide; Oocyte; Ooplasm; Transfer. |
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Thesaurus Nal: |
mitochondrial DNA. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/876985/1/OoplastMediatedDevelopmental.pdf
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Marc: |
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008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.rbmo.2010.10.011$2DOI
100 1 $aCHIARATTI, M. R.
245 $aOoplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.$h[electronic resource]
260 $c2011
520 $aOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes.
650 $amitochondrial DNA
653 $aEmbryo
653 $aEthidium bromide
653 $aOocyte
653 $aOoplasm
653 $aTransfer
700 1 $aFERREIRA, C. R.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aSANGALLI, J. R.
700 1 $aMESQUITA, L. G.
700 1 $aBALIEIRO, J. C. de C.
700 1 $aSMITH, L. C.
700 1 $aGARCIA, J. M.
700 1 $aMEIRELLES, F. V.
773 $tReproductive BioMedicine Online$gv. 22, n. 22, p. 172-183, 2011.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| 241. |  | BERNARDI, A. C. de C.; SEGNINI, A.; PRIMAVESI, O. M. A. S. P. R.; OLIVEIRA, P. P. A.; PEZZOPANE, J. R. M.; BERNDT, A.; BAYER, C.; MILORI, D. M. B. P.; SILVA, W. T. L. da; SIMOES, M. L.; MARTIN NETO, L. Increasing yield and carbon sequestration in a signalgrass pasture by liming and fertilization in São Carlos, São Paulo, Brazil. In: FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS. Recarbonizing global soils: a technical manual of recommended management practices. Rome: FAO, 2021. (Cropland, grassland, integrated systems and farming approaches: case studeis, v.4). Cap. 32. p.352-363.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Instrumentação; Embrapa Pecuária Sudeste. |
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| 242. | | SILVA, W. T. L. DA; HANEDA, R. N.; NOVAES, A. P. DE; MILORI, D. M. P.; MARTIN-NETO, L. SIMÕES, M. L.; FIALHO, L. L.; CAMPOS, J. B. DE; ANDERAÓS NETO, N.; NAJM FILHO, M.; ALMEIDA, L. C. P. DE; SILVEIRA, D. Humidification degree of fermentated urban residues determined by chemical and spectroscopic methods. In: INTERNATIONAL MEETING OF INTERNATIONAL HUMIC SUBSTANCES SOCIETY. -- IHSS, 12, São Pedro, SP, 2004. Humic substances and soil and water environment. Abstracts...[São Carlos], SP: Embrapa Instrumentação Agropecuária, 2004. p. 107. O nome correto de um dos autores é MILORI, D. M. B. P.| Biblioteca(s): Embrapa Instrumentação. |
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| 243. | | SILVA, W. T. L. da; HANEDA, R. N.; NOVAES, A. P. de; SIMOES, M. L.; FIALHO, L. L.; MILORI, D. M. B. P.; MARTIN-NETO, L.; CAMPOS, J. B. de ANDERÁOS NETO, N.; NAJM FILHO, M.; ALMEIDA, L. C. P. de; SILVEIRA, D. Humification degree of fermentated urban residues determined by chemical and spectroscopic methods. In: MARTIN-NETO, L.; MILORI, D.M.B.P.; SILVA, W.T.L. (Ed.). Humic substances and soil and water environment: International Meeting of IHSS, 12. São Carlos : Embrapa Instrumentação Agropecuária, 2004. p. 550-553. CLASSIFICAÇÃO CDD: 630| Biblioteca(s): Embrapa Instrumentação. |
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| 244. | | DIDONET, A. D.; FREITAS, A. A. de; MELLO, A. F. S.; EVANGELISTA, B. A.; GAVA, C. A. T.; BITTENCOURT, D. M. de C.; LIMA, D. de B.; TAVARES, E. D.; FIDALGO, E. C. C.; ALVES, E. M. P.; COUDEL, E. S.; CRUZ, F. T. da; DINIZ, J. D. de A. S.; CORREIA, J. R.; NOGUEIRA, J. D.; ESPINDOLA, J. A. A.; MEDEIROS, J. da C.; SCHWENGBER, J. E.; MORAIS, J. C. de; WOLFF, L. F.; ONOYAMA, M. M.; UDRY, M. C. F. V.; CORREIA, M. E. F.; VIDAL, M. C.; UZEDA, M. C.; PEREIRA, M. C. N.; PERDIGÃO, N. R. de O. F.; FACÓ, O.; JULIANO, R. S.; BRIENZA JUNIOR, S.; FAVARO, S. P.; VIOLA, T. H.; DIAS, T. A. B.; VIDAL, V. L.; GUEDES, V. G. F.; GUIMARÃES, V. P.; SILVA, W. T. L. da. Desafios de inovação para a agricultura familiar - estratégia para a agricultura familiar: visão de futuro rumo à inovação. In: BITTENCOURT, D. M. de C. (Ed.). Estratégias para a agricultura familiar: visão de futuro rumo à inovação. Brasília, DF: Embrapa, 2020. p. 257-289. (Texto para discussão, 49). Este capítulo traz os resultados do workshop Estratégia para Agricultura Familiar: visão de futuro rumo à inovação, realizado em Brasília, no período de 3 a 5 de outubro de 2017.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Agroenergia; Embrapa Amazônia Ocidental; Embrapa Arroz e Feijão; Embrapa Caprinos e Ovinos; Embrapa Clima Temperado; Embrapa Hortaliças; Embrapa Instrumentação; Embrapa Pantanal; Embrapa Pesca e Aquicultura; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido; Embrapa Solos. MenosEmbrapa Agrobiologia; Embrapa Agroenergia; Embrapa Amazônia Ocidental; Embrapa Arroz e Feijão; Embrapa Caprinos e Ovinos; Embrapa Clima Temperado; Embrapa Hortaliças; Embrapa Instrumentação; Embrapa Pantanal; Embrapa Pesca e Aquicultura... Mostrar Todas |
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